Milk Bases, Nucleosides and Nucleotides Quantification by HPLC Ref. #S1100-01
Quantity	Price*
1 sample (15 nucleobases, nucleosides, nucleotides and RNA)	€ 450.00 per one analysis For more samples ask for Quotation
* Pricing updated April 7^th, 2015 For more information on Infant Formula Nucleotide Analysis click here Download "Nucleotide Analysis Infant Formula"

HPLC analysis of Nucleotides in Infant Formula

The concentration of free nucleotides in human milk is significantly higher than in bovine milk. It was demonstrated that infants who received nucleotides in their formula were less likely to have diarrhea and also had better responses to vaccination, producing more antibodies and other immune cells (Carver et al., 1991; Pickering et al., 1998). Most milk formulas are supplemented with nucleotides to increase the concentration to a level that is similar to the concentration found in human milk.

NOVOCIB offers analytical service for quantification of dietary nucleotides in baby milk powder by UV-HPLC. This high performance liquid chromatography analysis of bases, nucleosides and nucleotides consists in:

Sample extraction by SPE procedure;
Run of extracted samples in ion-paired HPLC (Agilent 1100);
Bases, nucleosides and nucleotides quantification (peak area at 254nm, Agilent Software) Separation by ion-paired
Recovery study (every sample is analysed twice with and without added bases, nucleosides and nucleotides standards)
Delay: 5 working days

Example:

Nucleotides	Chemical class	mg/100g
Cytosine	Base	0.10
Uracyl	Base	0.38
Guanine	Base	2.80
Hypoxanthine	Base	0.00
Adenine	Base	0.00
Cytidine	Nucleoside	2.47
Uridine	Nucleoside	3.22
Guanosine	Nucleoside	0.72
Adenosine	Nucleoside	0.06
Inosine	Nucleoside	5.81
CMP	Cytidine monophosphate	9.81
UMP	Uridine monophosphate	4.36
GMP	Guanosine monophosphate	1.24
IMP	Inosine monophosphate	2.26
AMP	Adenosine monophosphate	4.91

References
A. Singhal (2008): Dietary nucleotides and fecal microbiota in formula-fed infants: a randomized controlled trial Am. J. Clin. Nutr. 87(6), 1785-1792
J. P. Schaller et al. (2007): Ribonucleotides: conditionally essential nutrients shown to enhance immune function and reduce diarrheal in infants Semin. Fetal Neonatal Med. 12(1), 35-44 & Erratum in Semin. Fetal Neonatal Med. 12(4), 326–328
A. Lerner and R. Shamir (2000): Nucleotides in infant nutrition: a must or an option? Isr. Med. Assoc. J. 2(10), 772-774
O. Brunser et al. (1994): ): Effects of dietary nucleotide supplementation on diarrhoeal disease in infants Acta Paediatr. 83(2), 188-191
J. D. Carver et al. (1991): ): Dietary nucleotide effects upon immune function in infants Paediatrics 88(2), 359-363
C. DeLucchi et al. (1987): ): Effects of dietary nucleotides on the fatty acid composition of erythrocyte membrane lipids in term infants J. Pediatr. Gastroenterol. Nutr. 6(4), 568-574
L. M. Janas and M. F. Picciano (1982): ): The nucleotide profile of human milk Pediatr. Res. 16(8), 659-662

Milk Bases, Nucleosides and Nucleotides Quantification by HPLC

HPLC analysis of Nucleotides in Infant Formula