Highly Pure Bacterial Luciferase (E.C.

from Photobacterium phosphoreum.

Ref. #E-Nov10

#REF Quantity Price
#E-Nov10-1 1 mg € 295.00 Inquiry
#E-Nov10-2 2 mg € 488.00 Inquiry
#E-Nov10-5 5 mg € 1106.00 Inquiry

Kit is provided in stable lyophilized form and shipped without dry ice

Bulk quantity available.

You can ask us for a quotation here or write at contact@novocib.com

Download NOVOCIB's Bacterial Luciferase

NOVOCIB's bacterial luciferase is purified from a Photobacterium phosphoreum strain isolated from squid by our team and selected for its brightest luminescence. The luxab gene was amplified by PCR and cloned. The sequences of cloned α and β subunits have shown 94% and 92% identity to P24113 and P12744 proteins of Photobacterium phosphoreum (SwissProt Entry).

Applications: Applications: In luminescent marine photobacteria, the production of light results from two successive reactions. The first one is catalyzed by the NAD(P)H-FMN oxidoreductase (EC, that produces FMNH2 acting as a substrate for the second reaction, which is catalyzed by a luciferase (EC to generate light in the presence of an aliphatic aldehyde and molecular oxygen. In the presence of limiting concentrations of NADH substrate, light intensity is proportional to NAD(P)H concentration. The coupling of bacterial luciferase to FMN-NAD(P)H oxidoreductase has been used to provide ultrasensitive analytical tools for the quantification of NAD(P)H and the substrates of NADH-, NADPH- dependent enzymes (e.g. glucose, lactate, malate, ethanol, sorbitol, oxaloacetate)(1).

Synonyms: Alkanal, reduced-FMN:oxygen oxidoreductase (1-hydroxylating, luminescing)

NOVOCIB 's Highly Pure Bacterial Luciferase can be used for NAD(P)H quantification or in dehydrogenase-coupled assays.

Lux gel photo
Activity: > 500,000 RLU per second per µg of protein in the presence of 10µM NADH and 3.5mU/ml FMNreductase (ref. E-Nov-8) as measured with an Optocomp 1 (Celsis) luminometer.
Purity controlled by 10% AA SDSPAGE
Lux Calibration Graph
Fig. 1. Calibration curves (log-log plot) for NADH obtained using Highly Pure Bacterial Luciferase (50µg/ml, NovoCIB, E-Nov 10). Assay condition: Luminescence signal (15-seconds) was measured after NADH addition (5µL) to 200µL-well containing 0.1M KH2PO4 pH=6.9, 0.02% dodecanal, 50µM FMN, 2mg/ml BSA. Reaction was followed in an Optocomp 1 (Celsis) luminometer.
ATP degradation Graph
(Scheme 1): Postmortem ATP degradation in muscle
References (with external links to PubMed)
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