Human Active Deoxycytidine Kinase Enzyme

NOVOCIB's human deoxycytidine kinase is an active and purified protein of 33kDa cloned by RT-PCR amplification of mRNA extracted from human hepatoma cells and expressed in E.coli.

Deoxycytidine kinase is responsible for the phosphorylation and activation of numerous nucleoside analogs used to treat cancer (e.g. cytarabine, gemcitabine, cladribine, and fludarabine) including nucleoside analogs of non-physiological L-chirality (e.g. 3TC, lamivudine, anti-HIV, and anti-hepatitis B agent).

For rapid evaluation of substrates properties of novel nucleoside analogues for human deoxycytidine kinase, see our PRECICE® dCK Phosphorylation Assay Kit

Human deoxycytidine kinase plays a key role in the salvage pathway of deoxynucleotides synthesis providing resting cells with deoxynucleotides for DNA repair and mitochondrial DNA synthesis. The enzyme has a broad substrate specificity and provides the phosphorylation of both purine and pyrimidine deoxynucleosides (e.g. deoxyadenosine (dA), deoxyguanosine (dG), deoxycytidine (dC)), and pyrimidine ribonucleoside cytidine (C).