Award
Dr Larissa Balakireva, CEO & Founder of NovoCIB, was awarded with the Trophy of
"Femmes en Or 2011, Femme de l'Innovation"
in September 2011
.

ITP pyrophosphohydrolase Assay Kit Principle

PRECICE® ITPase Assay Kit

Ref. #K0709-06-2
Size Price*
24 analyses (8 samples in triplicate) € 310.00
* Pricing updated April 28th, 2014.
Kit provided in stable lyophilized form and shipped without dry ice.

To buy ITPase Assay Kit click here or ask for Quotation


Download ITPase assay Protocol  
(User Manual)

One-step ITP pyrophosphatase assay

PRECICE® ITPase Assay kit provides the first non radioactive and continuous protocol for measurement of ITP pyrophosphatase activity (also known as inosine triphosphatase and inosine triphosphate pyrophosphohydrolase) in cellular lysates in a convenient 96-well plate format.

ITP pyrophosphatase, or ITPase (EC 3.6.1.19), is an intracellular enzyme that catalyzes the pyrophosphohydrolysis of ITP/dITP and xanthosine triphosphate to prevent unusual nucleoside triphosphates from accumulating in (deoxy) nucleoside triphosphate (d)NTP pools and being integrated into RNA and DNA.
This enzyme is encoded by the ITPA gene in mammals. Certain ITPA variants causing ITPase deficiency have been linked to adverse reactions to the immunosuppressive thiopurine drugs azathioprine and 6-mercaptopurine, and protection against ribavirin-induced anemia.


Validated

    Specific activitiy of ITP pyrophosphohydrolase in lysates of human erythrocytes measured by PRECICE® ITPase Assay kit

    ITP pyrophosphohydrolase activity in red blood cell lysate  
    RBC speciment 1 (n=2)
    94+/-3 nmol/hour/mg Hb
    RBC speciment 2 (n=2) 83+/-3 nmol/hour/mg Hb

Convenient

    Non radioactive
    Continuous
    No sample preparation required. Cell lysates are directly used for continuous monitoring of ITPA activity

Fast

    Simultaneous analysis of up to 12 samples in duplicate in 1 h

Principle
Inosine triphosphatase activity is measured as a rate of production of IMP with concomitant formation of NADH2 after one enzymatic reaction using highly active IMPDH. The formation of NADH2 is continuously monitored spectrophotometrically at 340nm.

Please, contact us for any questions.