Human cytosolic 5'-nucleotidase II

Human cytosolic 5'-nucleotidase II (cN-II) (E.C.3.1.3.5)

NOVOCIB'S human cytosolic IMP/GMP-specific 5'-nucleotidase/phosphotransferase II (cN-II) is a pure and active protein of 65kDa cloned by RT-PCR amplification of mRNA extracted from human hepatoma cells and expressed in E.coli. The sequence of the cloned NT5C2 gene (GenBank accession number P49902) was confirmed by DNA sequencing (100% identity).
Cytosolic 5'-nucleotidase II specifically catalyzes the dephosphorylation of 6-hydroxypurine nucleoside 5'-monophosphates (IMP, dIMP, GMP, dGMP) and regulates cellular pools of IMP and GMP. The enzyme also acts as a phosphotransferase catalyzing phosphate transfer from nucleoside monophosphates to nucleoside acceptors, preferentially inosine and deoxyinosine. Unlike other 5'-nucleotidases, cN-II is allosterically regulated by adenine/guanine nucleotides and 2,3-biphosphoglycerate.
In addition, cN-II phosphorylates antiviral and antitumor nucleoside analogues such as 2'3'-dideoxyinosine, carbovir, acyclovir, and ribavirin.

Synonyms: cytosolic 5'-nucleotidase/phosphotransferase, High Km 5'-nucleotidase (HKM-NT), cytosolic purine 5'-nucleotidase (purine 5'-NT), IMP/GMP-specific 5'-nucleotidase.

Download Human cytosolic 5'-nucleotidase II (User manual)

Assay condition:

• Temperature: 37°C
• Buffer: 100mM Tris-HCl pH 9
• Reagents: 100mM KCl, 20mM MgCl2, 0.5mM KH2PO4, 5mM DTT, etc.
• Phosphate donor: 5mM GMP
• Phosphate acceptor: 5mM inosine
• Method: Coupling to recombinant IMP-dehydrogenase (IMPDH, NovoCIB)

Assay condition:

• Temperature: 37°C
• Buffer: 50mM Tris-HCl pH7.6
• Reagents: 100mM KCl, 20mM MgCl2, 500µM KH2PO4, etc.
• Method: Coupling with purine nucleoside phosphorylase - xanthine dehydrogenase system.