Award
Dr Larissa Balakireva, CEO & Founder of NovoCIB, was awarded with the Trophy of
"Femmes en Or 2011, Femme de l'Innovation"
in September 2011
.

Deoxycytidine Kinase

Ref. #E-Nov3
Quantity Price*
250 mUnits
Supplied as 2 tubes of 125 mU of lyophilized enzyme
€ 295.00
400 mUnits
Supplied as 2 tubes of 200 mU of lyophilized enzyme
€ 415.00

* Pricing updated February 3rd, 2014

Provided in stable lyophilized form and shipped without dry ice
To order dCK click here
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Quotation

Human Deoxycytidine Kinase (dCK, EC 2.7.1.74)

NOVOCIB's human deoxycytidine kinase is an active and purified protein of 33kDa cloned by RT-PCR amplification of mRNA extracted from human hepatoma cells and expressed in E.coli.

Deoxycytidine kinase is responsible for the phosphorylation and activation of numerous nucleoside analogs used to treat cancer (e.g. cytarabine, gemcitabine, cladribine, and fludarabine) including nucleoside analogs of non-physiological L-chirality (e.g. 3TC, lamivudine, anti-HIV, and anti-hepatitis B agent).

For rapid evaluation of substrates properties of novel nucleoside analogues for human deoxycytidine kinase, see our PRECICE® dCK Phosphorylation Assay Kit

Human deoxycytidine kinase plays a key role in the salvage pathway of deoxynucleotides synthesis providing resting cells with deoxynucleotides for DNA repair and mitochondrial DNA synthesis. The enzyme has a broad substrate specificity and provides the phosphorylation of both purine and pyrimidine deoxynucleosides (e.g. deoxyadenosine (dA), deoxyguanosine (dG), deoxycytidine (dC)), and pyrimidine ribonucleoside cytidine (C).



Unit Definition: One unit of deoxycytidine kinase converts 1.0 µmole of deoxyinosine and ATP to dIMP and ADP per minute at pH 8 at 37°C, as measured by a coupled IMPDH enzyme system. (see dCK Assay Kit for further details).

Specific Activity: ≥ 0.5 unit/mg protein.

Purity: controlled by 12%AA SDS-PAGE.


 
 

References (with links to PubMed)
1. E. Sabini et al. (2003): Structure of human dCK suggests strategies to improve anticancer and antiviral therapy Nat. Struct. Biol. 10(7), 513-519
2. E. Sabini et al. (2008): Structural basis for substrate promiscuity of dCK J. Mol. Biol. 378(3), 607-621
3. E. Sabini et al. (2007): Structural basis for activation of the therapeutic l-nucleoside analogs 3TC and troxacitabine by human deoxycytidine kinase Nucleic Acids Res. 35(1), 186-192

Download the document "NovoCIB's Deoxycytidine kinase" 
Know more about our Nucleoside kinases catalogue.


Related Links
dCK Assay Kit
Nucleoside Kinases
ADK enzyme
CMK enzyme
cN-II enzyme
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