Continuous PNP Assay Kit

PRECICE^® PNP Assay kit provides the protocol for direct and continuous measurement of PNP activity in a convenient 96-well plate format.

Purine nucleoside phosphorylase deficiency is a rare human metabolic disorder which results in accumulation of toxic metabolites in T-cell lymphocytes and severe immunodeficiency. Purine nucleoside phosphorylase deficiency can be also associated with neurologic symptoms, including mental retardation and muscle spasticity, and increased risk of autoimmune disorders.

Principle
PNP activity is measured as a rate of production of hypoxanthine which is directly oxidized by XDH enzyme with concomitant reduction of NAD+ to NADH measurable by absorbance at 340nm. The assay is developed for measuring PNP activity in vitro or in cell lysates.

Validated

• Specific activitiy of PNP in lysates of human erythrocytes measured by PRECICE^® PNP Assay kit is close to previously published data

		RBC speciment 1 (n=3) 985 +/-38 nmols/hour/mg Hb RBC speciment 2 (n=3) 1037+/-30 nmols/hour/mg Hb RBC speciment 3 (n=3) 1132+/-56 nmols/hour/mg Hb RBC speciment 4 (n=3) 911+/-12 nmols/hour/mg Hb Published data Rubocki RG et al (2001) Blood, 97, 3 1336+/-441nmols/hour/mg Hb

Fast

• 20 minutes per sample