Award
Dr Larissa Balakireva, CEO & Founder of NovoCIB, was awarded with the Trophy of
"Femmes en Or 2011, Femme de l'Innovation"
in September 2011
.

PNP Assay Kit Principle

PRECICE® PNP Assay Kit

Ref. #K0709-03-2
Size Price*
24 analyses (8 samples in triplicate) € 315.00

* Pricing updated January 24th, 2014.
Kit provided in stable lyophilized form and shipped without dry ice.

Limited number of samples avaible for FREE

To buy PNP Assay Kit click here or ask for Quotation

Download PNP in vitro assay protocol  
(User Manual)

Continuous PNP Assay Kit

PRECICE® PNP Assay kit provides the protocol for direct and continuous measurement of PNP activity in a convenient 96-well plate format.

Purine nucleoside phosphorylase deficiency is a rare human metabolic disorder which results in accumulation of toxic metabolites in T-cell lymphocytes and severe immunodeficiency. Purine nucleoside phosphorylase deficiency can be also associated with neurologic symptoms, including mental retardation and muscle spasticity, and increased risk of autoimmune disorders.

Principle
PNP activity is measured as a rate of production of hypoxanthine which is directly oxidized by XDH enzyme with concomitant reduction of NAD+ to NADH measurable by absorbance at 340nm. The assay is developed for measuring PNP activity in vitro or in cell lysates.

Validated

    Specific activitiy of PNP in lysates of human erythrocytes measured by PRECICE® PNP Assay kit is close to previously published data

    PNP activity in red blood cell lysate  
    RBC speciment 1 (n=3)
    985 +/-38 nmols/hour/mg Hb
    RBC speciment 2 (n=3) 1037+/-30 nmols/hour/mg Hb
    RBC speciment 3 (n=3) 1132+/-56 nmols/hour/mg Hb
    RBC speciment 4 (n=3) 911+/-12 nmols/hour/mg Hb

    Published data
    Rubocki RG et al (2001) Blood, 97, 3

    1336+/-441nmols/hour/mg Hb

Fast

    20 minutes per sample

Please, contact us for any questions.

 

Related Links
PNP enzyme
XDH enzyme
Continuous Phosphatase Assay