Award
Dr Larissa Balakireva, CEO & Founder of NovoCIB, was awarded with the Trophy of
"Femmes en Or 2011, Femme de l'Innovation"
in September 2011
.

Nucleoside Analogues Phosphorylation

Nucleoside analogues are cornerstones of current anticancer and antiviral therapies. Antiviral nucleoside analogs inhibit viral replication, whereas anticancer nucleosides inhibit replication of cellular DNA and repair. Nucleoside analogs are inactive prodrugs and their therapeutic efficacy depends on their intracellular phosphorylation.

Adenosine Kinase (ADK)
Deoxycytidine Kinase (dCK)
Natural substrates
Adenosine
Inosine
Deoxycytidine
Cytidine
Deoxyadenosine
Deoxyguanosine
Nucleoside analogues
Ribavirine
Tubercidin
Mizoribine
Cladribine
Fludarabine
Gemcitabine (dFdC)
Lamivudine
Aracytidine (araC)
Fluorodeoxyuridine

Cellular deoxycytidine kinase (dCK) provides the first step of activation of highly effective anti-cancer and anti-viral drugs, such as 1-β-D-arabinofuranosylcytosine (araC, aracytidine), 2’,2’difluorodeoxycytidine (dFdC, gemcitabine), β-D-2’3’-dideoxycytidine (ddC). dCK kinase phosphorylates alsounnatural L-nucleosides (e.g., β-L-2’3’-dideoxy-3’thiacytidine, L-SSdC, 3-TC or lamividune).

Cellular adenosine kinase (ADK) provides phosphorylation of ribavirin (1-β-D-ribofuranosyl-1,2,4-triazole-3-carboxamide), purine nucleoside analogue with a broad-spectrum antiviral activity.

For rapid evaluation of substrate specificy of novel anticancer and antiviral nucleoside analogues NOVOCIB offers
- dCK Phosphorylation Assay Kit
- ADK Phosphorylation Assay Kit.
These kits allow rapid characterisation of first phosphorylation step of ribonucleoside and deoxynucleoside analogues in comparison with phosphorylation properties of known nucleoside drugs and provides the rational basis for selection of analogues of better therapeutic potential.

For HTS evaluation of ADK activity in lysates, NOVOCIB also supplies ADK Assay Kit.


Related Links
ADK Assay Kit
ADK enzyme
dCK Assay Kit
dCK enzyme