Measure nucleotides to assess food quality

Since seafood and fish are highly perishable products, the quality of raw material is of primary concern for seafood processing industry. While sensory analysis allows rapid evaluation of the quality of whole wet fish, the assessment of the quality of frozen fish, or that of frozen blocks of fillets or crumbed seafood products remains more difficult.

PRECICE^® Nucleotides Assay Kit is a first enzymatic assay for spectrophotometric measurement of three major ATP degradation products. The enzymes provided with kit allow specific conversion of three major ATP catabolites - IMP, inosine (Ino) and hypoxanthine (Hx) to NADH₂. The quantification of nucleotides is done by measuring sample absorbance at 340nm.

Rapid & Precise

• Enzymatic reactions take 30 min
• R²=0.89 (IMP, HPLC); R²=0.91 (inosine, HPLC); R²=0.91 (hypoxanthine, HPLC);
• R²=0.88 found between H-value measured with PRECICE^® Nucleotides Assay Kit and TMA/ABVT in whiting fillets (11 samples)

Wide Application Range

• Frozen, fresh, stored under modified atmosphere, smoked, sterilized and canned products

Download:
User Manual  "PRECICE® Nucleotides Assay Kit" for cuvette spectrophotometer
User Manual  "PRECICE® Nucleotides Assay Kit" for plate reader