PRECICE® AMP Deaminase Assay Kit
Ref. #K0709-05-2 |
Size |
Price* |
12 analyses (6 ml reaction buffer) |
€ 210.00 |
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24 analyses (12 ml reaction buffer) |
€ 410.00 |
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* Pricing updated March 3rd, 2022.
Kit provided in stable lyophilized form and shipped without dry ice.
To buy AMPD Assay Kit click here or ask for Quotation
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Non radioactive AMP Deaminase assay
PRECICE® AMP Deaminase Assay kit provides the first non radioactive and one-step protocol for measurement of AMP Deaminase activity in cellular lysates in a convenient 96-well plate format.
Validated
• Specific activitiy of AMP Deaminase in lysates of human erythrocytes measured by PRECICE® AMP Deaminase Assay kit
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RBC speciment 1 (n=2) |
216+/-1 nmol/hour/mg Hb |
RBC speciment 2 (n=2) |
226+/-5 nmol/hour/mg Hb |
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Convenient
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Non radioactive
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Continuous
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No sample preparation required. Cell lysates are directly used for continuous monitoring
of AMP Deaminase activity
Fast
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Simultaneous analysis of up to 12 samples in duplicate in 1 h
Principle
AMP Deaminase activity is measured as a rate of production of IMP with concomitant formation of NADH2 after one enzymatic reaction using highly active IMPDH. The formation of NADH2 is continuously monitored spectrophotometrically at 340nm.
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